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KMID : 0380020210360040279
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Korean Journal of Biotechnology and Bioengineering
2021 Volume.36 No. 4 p.279 ~ p.284
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Apoptotic Effects from Hexane fractions of Lysimachiae foenum-graeci Herba through Regulating Akt/Mdm2 Signaling Pathways in HCT116 Colon Cancer Cells
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Seo Ji-Won
Lee Hyo-Jae
Lee Chang-Yeol
Kim Sang-Yong
Kim Young-Min
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Abstract
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Lysimachiae foenum-graeci Herba is a shade-dried whole plants of the Lysimachia foenum-graecum Hance and is well known to have anti-oxidant, anti-obesity and antibacterial effects. Accordingly, in this study, we assessed the apoptotic effects of hexane fractions from Lysimachiae foenum-graecum (LFH) on HCT116 Human colon cancer cells, and to confirm whether this effects are due to the regulation of Akt/Mdm2 signal pathways. As a result of MTT assay, LFH showed cell proliferation inhibitory and cytotoxic effects of HCT116 cells, and confirmed through Annexin V & Dead Cell assay to the staining rate of phosphatidylserine (PS) expressed by apoptosis. In addition, it was confirmed through Hoechst 33342 assay that the fluorescence caused by apoptosis progression and apoptosis bodies were caused by apoptosis induction of HCT116 cells by LFH. And it was confirmed through Western blotting that treatment of LFH induced inhibition of p-Akt, p-Mdm2, Bcl-2, and Pro-caspase 3 and activation of p-AMPK, p53, Bak, Bax, and Cleaved-caspase 3. Furthermore, it was confirmed that the apoptosis induction effect by LFH was caused by Akt/Mdm2 pathway regulation through Akt inhibitor (LY294002) and Mdm2 inhibitor (Nutlin-3) treatment. In conclusion, it was confirmed that LFH induces apoptosis through Akt/Mdm2 signaling pathway in HCT116 Human colon cancer cells.
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KEYWORD
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Akt/Mdm2 pathway, anti-cancer, apoptosis, HCT116 human colon cancer, Lysimachiae foenum-graeci Herba
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